http://www.idkfa.com/v3 idkfa: syndicated http://www.idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=2023 Fucking around with the machine as we speak, setting a new baseline because the lasers are all gone crazy shit or something. My boss has been beating on the various filters for like the last 3 hours to get shit back where it should be. MrFood@idkfa.com (MrFood) Mercy General Thu, 03 Feb 2011 14:39:13 -0900 http://idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=2023 http://www.idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=2018 Like I said, an art! And daaaaaaaaaamn. I didn't know they were that expensive! Scrotor@idkfa.com (Scrotor) Mercy General Thu, 03 Feb 2011 14:28:58 -0900 http://idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=2018 http://www.idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=1992 Its just nerve racking to be screwing around on a machine that costs about $800k. To constantly be worrying that any false move and the thing will self destruct or some laser will fall out of alignment and the whole lab will be screwed until the maintenance guy can come around and fix the damn thing. Plus its in such high demand that ever time I'm ever on the infernal machine I get at least two requests from other people in the lab to see if I need the whole amount of time I have booked, and can they jump on early if they can. Then the data analysis is mind bogglingly complex and just hurts the brain after only an hour or two of looking at it. The individual scatter properties of different cell populations and different cell makers, and some cells that have various makers that you have to distinguish. Plus you have to hope to any god you choose that you even stained correctly in the first place so that any of this shit can actually make any sense. I have yet to have a single GOOD day of running flow. MrFood@idkfa.com (MrFood) Mercy General Tue, 01 Feb 2011 18:30:01 -0900 http://idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=1992 http://www.idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=1979 It's an art, really... Scrotor@idkfa.com (Scrotor) Mercy General Mon, 31 Jan 2011 02:19:40 -0900 http://idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=1979 http://www.idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=1969 Flow cytometry is like giving satan an old fashioned. Your arms even hurt after your done. Beer is going to taste so good when this day is over. MrFood@idkfa.com (MrFood) Mercy General Fri, 28 Jan 2011 17:02:18 -0900 http://idkfa.com/v3/v_thread.php?thread_id=1969&msg_id=1969